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Scientific Overview Research Interest Summary Principal Investigators    Yuri Bushkin, Ph.D.
   Theresa Chang, Ph.D.
   Neeraj Chauhan, Ph.D.
   Véronique Dartois, Ph.D.
   Karl Drlica, Ph.D.
   David Dubnau, Ph.D.
   Eliseo A. Eugenin, Ph.D.
   Marila Gennaro, M.D.
   Gilla Kaplan, Ph.D.
   Fred Kramer, Ph.D.
   Barry Kreiswirth, Ph.D.
   Min Lu, Ph.D.
   Leonard Mindich, Ph.D.
   Arkady Mustaev, Ph.D.
   David Perlin, Ph.D.
   Richard Pine, Ph.D.
   Abraham Pinter, Ph.D.
   Marcela Rodriguez, Ph.D.
   Issar Smith, Ph.D.
   Patricia Soteropoulos, Ph.D.
   Sanjay Tyagi, Ph.D.
   Chaoyang Xue, Ph.D.
   Xilin Zhao, Ph.D.

   Research Faculty
   Eugenie Dubnau, Ph.D.
   Jeanette Hahn, Ph.D.
   Salvatore Marras, Ph.D.
   Lanbo Shi, Ph.D.
   Alicia Solórzano, Ph.D.

Junior Faculty Members Emeritus Faculty Recent Publications
 
Barry Kreiswirth, Ph.D.

Research Summary  |  Selected Publications  |  C.V.
 

Public Health Research Institute Center
New Jersey Medical School - Rutgers, The State University of New Jersey
225 Warren Street
Newark, New Jersey 07103

Phone: (973) 854-3240
e-mail: kreiswba@njms.rutgers.edu


Research Summary

Dr. Kreiswirth is the founding director of the PHRI TB Center, a Professor of Medicine at Rutgers and has an adjunct faculty position in the Department of Medicine at New York University, is a senior lecturer at the Mailman School of Public Health at Columbia University and a visiting scientist at the Museum of Natural History. Dr Kreiswirth joined PHRI as a graduate student in 1978 in Dr. Richard Novick’s laboratory working on the molecular biology of Staphylococcus aureus and ultimately cloning and characterizing the genetic determinant responsible for toxic shock syndrome.

In 1992, in response to the New York City tuberculosis outbreaks, the PHRI TB Center was established under Dr. Kreiswirth’s direction as a genotyping laboratory to study the molecular epidemiology of tuberculosis. The center characterized the highly multidrug resistant strain “W” and beginning in 1992 it has genetically characterized over 27,000 M. tuberculosis isolates from global sources. Since its inception, the Center has worked closely with the Centers for Disease Control and Prevention and the New York City and New Jersey Departments of Health to integrate the tools of molecular biology with tuberculosis control efforts. The PHRI TB Center has extended its molecular epidemiological collaborations to global tuberculosis “hotspots” including the Russian prisons, the gold mines in Johannesburg and the XDR outbreak in KwaZulu-Natal, South Africa. Common to all studies is the characterization of drug resistance mutations and correlating phenotypic susceptibility assays with genetic alterations. The molecular epidemiological studies have created a rich genotypic database that has identified highly successful clones that have caused extensive disease throughout the United States and understanding their predominance and their microevolution has become a major effort in the laboratory.

The Kreiswirth laboratory is also focused on nosocomial infections and the emergence of antibiotic resistance and the research aims include the development of rapid genotyping methods for both molecular epidemiologic studies and to advance infection control strategies. The spa typing of S. aureus using the eGenomics analytical software has become a standard method to genotype the species to unravel suspected outbreaks and to determine phylogenetic relatedness. These studies have now established a biological foundation to extend the resolution of genotyping using whole genome sequencing and creating large comparative genomic databases. This work is now ongoing in collaboration with bioinformaticians and curators at the Sackler Institute of Comparative Genomics at the American Museum of Natural History.

The experiences in genotyping M. tuberculosis and S. aureus were brought to the emerging problem of carbapenem resistant Enterobacteriaceae which have rapidly spread across the New York Metropolitan area and which challenge both diagnostic and treatment protocols. In response, the PHRI TB Center has developed rapid molecular beacon-based genotypic assays to both identify and distinguish the predominant carbapenem resistant Klebsiella pneumoniae ST258 and to determine the genetic basis of its resistance. In parallel with the genomic studies to study the phylogeny of S. aureus, we are currently amassing genomic data for K. pneumoniae and the resistance plasmids they harbor to create comparative sequence databases that will be “mined” to create diagnostic platforms and targets for drug and vaccine development.




Selected Publications

Mathema B, Kurepina N, Yang G, Shashkina E, Manca C, Mehaffy C, Bielefeldt-Ohmann H, Ahuja S, Fallows DA, Izzo A, Bifani P, Dobos K, Kaplan G, Kreiswirth BN (2012) Epidemiologic Consequences of Microvariation in Mycobacterium tuberculosis. J Infect Dis. PMI: 22315279

Chen L, Shopsin B, Zhao Y, Smyth D, Wasserman GA, Fang C, Liu L, Kreiswirth BN (2012) A real-time nucleic acid sequence-based amplification (NASBA) assay for rapid detection and quantification of agr functionality in clinical Staphylococcus aureus isolates. J Clin Microbiol. PMI: 22219302

Tenover FC, Tickler IA, Goering RV, Kreiswirth BN, Mediavilla JR, Persing DH (2011) Characterization of Nares and Blood Culture Isolates of Methicillin-resistant Staphylococcus aureus from Patients in United States Hospitals. Antimicrob Agents Chemother. PMI: 22155818

Rosenthal ME, Dever LL, Moucha CS, Chavda KD, Otto M, Kreiswirth BN (2011) Molecular characterization of an early invasive Staphylococcus epidermidis prosthetic joint infection. Microb Drug Resist 17: 345-350. PMI: 21510745

Perri BR, Proops D, Moonan PK, Munsiff SS, Kreiswirth BN, Goranson C, Ahuja SD (2011) Mycobacterium tuberculosis cluster with developing drug resistance, New York, New York, USA, 2003-2009. Emerg Infect Dis 17: 372-378. PMI: 21392426

Patel G, Jenkins SG, Mediavilla JR, Kreiswirth BN, Radbill B, Salgado CD, Calfee DP (2011) Clinical and molecular epidemiology of methicillin-resistant Staphylococcus aureus among patients in an ambulatory hemodialysis center. Infect Control Hosp Epidemiol 32: 881-888. PMI: 21828968

Hu Y, Mathema B, Jiang W, Kreiswirth B, Wang W, Xu B (2011) Transmission pattern of drug-resistant tuberculosis and its implication for tuberculosis control in eastern rural China. PLoS One 6: e19548. PMI: 21589863

DeLeo FR, Kennedy AD, Chen L, Bubeck Wardenburg J, Kobayashi SD, Mathema B, Braughton KR, Whitney AR, Villaruz AE, Martens CA, Porcella SF, McGavin MJ, Otto M, Musser JM, Kreiswirth BN (2011) Molecular differentiation of historic phage-type 80/81 and contemporary epidemic Staphylococcus aureus. Proc Natl Acad Sci U S A 108: 18091-18096. PMI: 22025717

Chen L, Mediavilla JR, Endimiani A, Rosenthal ME, Zhao Y, Bonomo RA, Kreiswirth BN (2011) Multiplex real-time PCR assay for detection and classification of Klebsiella pneumoniae carbapenemase gene (bla KPC) variants. J Clin Microbiol 49: 579-585. PMI: 21123529

Chen L, Chavda KD, Mediavilla JR, Jacobs MR, Levi MH, Bonomo RA, Kreiswirth BN (2011) Partial Excision of blaKPC from Tn4401 in Carbapenem-Resistant Klebsiella pneumoniae. Antimicrob Agents Chemother. PMI: 22203593

Chatterjee SS, Chen L, Joo HS, Cheung GY, Kreiswirth BN, Otto M (2011) Distribution and regulation of the mobile genetic element-encoded phenol-soluble modulin PSM-mec in methicillin-resistant Staphylococcus aureus. PLoS One 6: e28781. PMI: 22174895

Bush K, Courvalin P, Dantas G, Davies J, Eisenstein B, Huovinen P, Jacoby GA, Kishony R, Kreiswirth BN, Kutter E, Lerner SA, Levy S, Lewis K, Lomovskaya O, Miller JH, Mobashery S, Piddock LJ, Projan S, Thomas CM, Tomasz A, Tulkens PM, Walsh TR, Watson JD, Witkowski J, Witte W, Wright G, Yeh P, Zgurskaya HI (2011) Tackling antibiotic resistance. Nat Rev Microbiol 9: 894-896. PMI: 22048738

Shopsin B, Eaton C, Wasserman GA, Mathema B, Adhikari RP, Agolory S, Altman DR, Holzman RS, Kreiswirth BN, Novick RP (2010) Mutations in agr do not persist in natural populations of methicillin-resistant Staphylococcus aureus. J Infect Dis 202: 1593-1599. PMI: 20942648

Kobayashi SD, Braughton KR, Palazzolo-Ballance AM, Kennedy AD, Sampaio E, Kristosturyan E, Whitney AR, Sturdevant DE, Dorward DW, Holland SM, Kreiswirth BN, Musser JM, DeLeo FR (2010) Rapid neutrophil destruction following phagocytosis of Staphylococcus aureus. J Innate Immun 2: 560-575. PMI: 20587998

Kennedy AD, Porcella SF, Martens C, Whitney AR, Braughton KR, Chen L, Craig CT, Tenover FC, Kreiswirth BN, Musser JM, DeLeo FR (2010) Complete nucleotide sequence analysis of plasmids in strains of Staphylococcus aureus clone USA300 reveals a high level of identity among isolates with closely related core genome sequences. J Clin Microbiol 48: 4504-4511. PMI: 20943864

Elie-Turenne MC, Fernandes H, Mediavilla JR, Rosenthal M, Mathema B, Singh A, Cohen TR, Pawar KA, Shahidi H, Kreiswirth BN, Deitch EA (2010) Prevalence and characteristics of Staphylococcus aureus colonization among healthcare professionals in an urban teaching hospital. Infect Control Hosp Epidemiol 31: 574-580. PMI: 20426580

Deleo FR, Otto M, Kreiswirth BN, Chambers HF (2010) Community-associated meticillin-resistant Staphylococcus aureus. Lancet 375: 1557-1568. PMI: 20206987

Chen L, Mediavilla JR, Smyth DS, Chavda KD, Ionescu R, Roberts RB, Robinson DA, Kreiswirth BN (2010) Identification of a novel transposon (Tn6072) and a truncated staphylococcal cassette chromosome mec element in methicillin-resistant Staphylococcus aureus ST239. Antimicrob Agents Chemother 54: 3347-3354. PMI: 20479198

Chakravorty S, Aladegbami B, Thoms K, Lee JS, Lee EG, Rajan V, Cho EJ, Kim H, Kwak H, Kurepina N, Cho SN, Kreiswirth B, Via LE, Barry CE, 3rd, Alland D (2010) Rapid detection of fluoroquinolone-resistant and heteroresistant Mycobacterium tuberculosis by use of sloppy molecular beacons and dual melting-temperature codes in a real-time PCR assay. J Clin Microbiol 49: 932-940. PMI: 21191047

Zhao Y, Park S, Kreiswirth BN, Ginocchio CC, Veyret R, Laayoun A, Troesch A, Perlin DS (2009) Rapid real-time nucleic Acid sequence-based amplification-molecular beacon platform to detect fungal and bacterial bloodstream infections. J Clin Microbiol 47: 2067-2078. PMI: 19403758

Xiong YQ, Fowler VG, Yeaman MR, Perdreau-Remington F, Kreiswirth BN, Bayer AS (2009) Phenotypic and genotypic characteristics of persistent methicillin-resistant Staphylococcus aureus bacteremia in vitro and in an experimental endocarditis model. J Infect Dis 199: 201-208. PMI: 19086913

Shet A, Mathema B, Mediavilla JR, Kishii K, Mehandru S, Jeane-Pierre P, Laroche M, Willey BM, Kreiswirth N, Markowitz M, Kreiswirth BN (2009) Colonization and subsequent skin and soft tissue infection due to methicillin-resistant Staphylococcus aureus in a cohort of otherwise healthy adults infected with HIV type 1. J Infect Dis 200: 88-93. PMI: 19463064

Queck SY, Khan BA, Wang R, Bach TH, Kretschmer D, Chen L, Kreiswirth BN, Peschel A, Deleo FR, Otto M (2009) Mobile genetic element-encoded cytolysin connects virulence to methicillin resistance in MRSA. PLoS Pathog 5: e1000533. PMI: 19649313

Middelkoop K, Bekker LG, Mathema B, Shashkina E, Kurepina N, Whitelaw A, Fallows D, Morrow C, Kreiswirth B, Kaplan G, Wood R (2009) Molecular epidemiology of Mycobacterium tuberculosis in a South African community with high HIV prevalence. J Infect Dis 200: 1207-1211. PMI: 19764885

Chen L, Mediavilla JR, Oliveira DC, Willey BM, de Lencastre H, Kreiswirth BN (2009) Multiplex real-time PCR for rapid Staphylococcal cassette chromosome mec typing. J Clin Microbiol 47: 3692-3706. PMI: 19726600

Bifani P, Kurepina N, Mathema B, Wang XM, Kreiswirth B (2009) Genotyping of Mycobacterium tuberculosis clinical isolates using IS6110-based restriction fragment length polymorphism analysis. Methods Mol Biol 551: 173-188. PMI: 19521875

Sinsimer D, Huet G, Manca C, Tsenova L, Koo MS, Kurepina N, Kana B, Mathema B, Marras SA, Kreiswirth BN, Guilhot C, Kaplan G (2008) The phenolic glycolipid of Mycobacterium tuberculosis differentially modulates the early host cytokine response but does not in itself confer hypervirulence. Infect Immun 76: 3027-3036. PMI: 18443098

Shopsin B, Drlica-Wagner A, Mathema B, Adhikari RP, Kreiswirth BN, Novick RP (2008) Prevalence of agr dysfunction among colonizing Staphylococcus aureus strains. J Infect Dis 198: 1171-1174. PMI: 18752431

Olsen RJ, Burns KM, Chen L, Kreiswirth BN, Musser JM (2008) Severe necrotizing fasciitis in a human immunodeficiency virus-positive patient caused by methicillin-resistant Staphylococcus aureus. J Clin Microbiol 46: 1144-1147. PMI: 18199782

Noto MJ, Kreiswirth BN, Monk AB, Archer GL (2008) Gene acquisition at the insertion site for SCCmec, the genomic island conferring methicillin resistance in Staphylococcus aureus. J Bacteriol 190: 1276-1283. PMI: 18083809

Mathema B, Mediavilla J, Kreiswirth BN (2008) Sequence analysis of the variable number tandem repeat in Staphylococcus aureus protein A gene: spa typing. Methods Mol Biol 431: 285-305. PMI: 18287764

Mathema B, Kurepina N, Fallows D, Kreiswirth BN (2008) Lessons from molecular epidemiology and comparative genomics. Semin Respir Crit Care Med 29: 467-480. PMI: 18810681

Kennedy AD, Otto M, Braughton KR, Whitney AR, Chen L, Mathema B, Mediavilla JR, Byrne KA, Parkins LD, Tenover FC, Kreiswirth BN, Musser JM, DeLeo FR (2008) Epidemic community-associated methicillin-resistant Staphylococcus aureus: recent clonal expansion and diversification. Proc Natl Acad Sci U S A 105: 1327-1332. PMI: 18216255

Bifani P, Mathema B, Kurepina N, Shashkina E, Bertout J, Blanchis AS, Moghazeh S, Driscoll J, Gicquel B, Frothingham R, Kreiswirth BN (2008) The evolution of drug resistance in Mycobacterium tuberculosis: from a mono-rifampin-resistant cluster into increasingly multidrug-resistant variants in an HIV-seropositive population. J Infect Dis 198: 90-94. PMI: 18498237

Fowler VG, Jr., Nelson CL, McIntyre LM, Kreiswirth BN, Monk A, Archer GL, Federspiel J, Naidich S, Remortel B, Rude T, Brown P, Reller LB, Corey GR, Gill SR (2007) Potential associations between hematogenous complications and bacterial genotype in Staphylococcus aureus infection. J Infect Dis 196: 738-747. PMI: 17674317

Burlak C, Hammer CH, Robinson MA, Whitney AR, McGavin MJ, Kreiswirth BN, Deleo FR (2007) Global analysis of community-associated methicillin-resistant Staphylococcus aureus exoproteins reveals molecules produced in vitro and during infection. Cell Microbiol 9: 1172-1190. PMI: 17217429

Weese JS, Dick H, Willey BM, McGeer A, Kreiswirth BN, Innis B, Low DE (2006) Suspected transmission of methicillin-resistant Staphylococcus aureus between domestic pets and humans in veterinary clinics and in the household. Vet Microbiol 115: 148-155. PMI: 16464540

Weese JS, Caldwell F, Willey BM, Kreiswirth BN, McGeer A, Rousseau J, Low DE (2006) An outbreak of methicillin-resistant Staphylococcus aureus skin infections resulting from horse to human transmission in a veterinary hospital. Vet Microbiol 114: 160-164. PMI: 16384660

Voyich JM, Otto M, Mathema B, Braughton KR, Whitney AR, Welty D, Long RD, Dorward DW, Gardner DJ, Lina G, Kreiswirth BN, DeLeo FR (2006) Is Panton-Valentine leukocidin the major virulence determinant in community-associated methicillin-resistant Staphylococcus aureus disease? J Infect Dis 194: 1761-1770. PMI: 17109350

Gutacker MM, Mathema B, Soini H, Shashkina E, Kreiswirth BN, Graviss EA, Musser JM (2006) Single-nucleotide polymorphism-based population genetic analysis of Mycobacterium tuberculosis strains from 4 geographic sites. J Infect Dis 193: 121-128. PMI: 16323140

Driver CR, Macaraig M, McElroy PD, Clark C, Munsiff SS, Kreiswirth B, Driscoll J, Zhao B (2006) Which patients' factors predict the rate of growth of Mycobacterium tuberculosis clusters in an urban community? Am J Epidemiol 164: 21-31. PMI: 16641308

Clark CM, Driver CR, Munsiff SS, Driscoll JR, Kreiswirth BN, Zhao B, Ebrahimzadeh A, Salfinger M, Piatek AS, Abdelwahab J (2006) Universal genotyping in tuberculosis control program, New York City, 2001-2003. Emerg Infect Dis 12: 719-724. PMI: 16704826

Weese JS, Archambault M, Willey BM, Hearn P, Kreiswirth BN, Said-Salim B, McGeer A, Likhoshvay Y, Prescott JF, Low DE (2005) Methicillin-resistant Staphylococcus aureus in horses and horse personnel, 2000-2002. Emerg Infect Dis 11: 430-435. PMI: 15757559

Voyich JM, Braughton KR, Sturdevant DE, Whitney AR, Said-Salim B, Porcella SF, Long RD, Dorward DW, Gardner DJ, Kreiswirth BN, Musser JM, DeLeo FR (2005) Insights into mechanisms used by Staphylococcus aureus to avoid destruction by human neutrophils. J Immunol 175: 3907-3919. PMI: 16148137

Sinsimer D, Leekha S, Park S, Marras SA, Koreen L, Willey B, Naidich S, Musser KA, Kreiswirth BN (2005) Use of a multiplex molecular beacon platform for rapid detection of methicillin and vancomycin resistance in Staphylococcus aureus. J Clin Microbiol 43: 4585-4591. PMI: 16145111

Said-Salim B, Mathema B, Braughton K, Davis S, Sinsimer D, Eisner W, Likhoshvay Y, Deleo FR, Kreiswirth BN (2005) Differential distribution and expression of Panton-Valentine leucocidin among community-acquired methicillin-resistant Staphylococcus aureus strains. J Clin Microbiol 43: 3373-3379. PMI: 16000462

Kurepina N, Likhoshvay E, Shashkina E, Mathema B, Kremer K, van Soolingen D, Bifani P, Kreiswirth BN (2005) Targeted hybridization of IS6110 fingerprints identifies the W-Beijing Mycobacterium tuberculosis strains among clinical isolates. J Clin Microbiol 43: 2148-2154. PMI: 15872234

Koreen L, Ramaswamy SV, Naidich S, Koreen IV, Graff GR, Graviss EA, Kreiswirth BN (2005) Comparative sequencing of the serine-aspartate repeat-encoding region of the clumping factor B gene (clfB) for resolution within clonal groups of Staphylococcus aureus. J Clin Microbiol 43: 3985-3994. PMI: 16081940

Geng EH, Kreiswirth BN, Burzynski J, Schluger NW (2005) Transmission trends for human immunodeficiency virus associated tuberculosis in New York City. Int J Tuberc Lung Dis 9: 661-666. PMI: 15971394

Geng E, Kreiswirth B, Burzynski J, Schluger NW (2005) Clinical and radiographic correlates of primary and reactivation tuberculosis: a molecular epidemiology study. JAMA 293: 2740-2745. PMI: 15941803

Cespedes C, Said-Salim B, Miller M, Lo SH, Kreiswirth BN, Gordon RJ, Vavagiakis P, Klein RS, Lowy FD (2005) The clonality of Staphylococcus aureus nasal carriage. J Infect Dis 191: 444-452. PMI: 15633104

Shopsin B, Zhao X, Kreiswirth BN, Tillotson GS, Drlica K (2004) Are the new quinolones appropriate treatment for community-acquired methicillin-resistant Staphylococcus aureus? Int J Antimicrob Agents 24: 32-34. PMI: 15225857

Reed MB, Domenech P, Manca C, Su H, Barczak AK, Kreiswirth BN, Kaplan G, Barry CE, 3rd (2004) A glycolipid of hypervirulent tuberculosis strains that inhibits the innate immune response. Nature 431: 84-87. PMI: 15343336

Post FA, Willcox PA, Mathema B, Steyn LM, Shean K, Ramaswamy SV, Graviss EA, Shashkina E, Kreiswirth BN, Kaplan G (2004) Genetic polymorphism in Mycobacterium tuberculosis isolates from patients with chronic multidrug-resistant tuberculosis. J Infect Dis 190: 99-106. PMI: 15195248

O'Brien FG, Lim TT, Chong FN, Coombs GW, Enright MC, Robinson DA, Monk A, Said-Salim B, Kreiswirth BN, Grubb WB (2004) Diversity among community isolates of methicillin-resistant Staphylococcus aureus in Australia. J Clin Microbiol 42: 3185-3190. PMI: 15243080

Milan SJ, Hauge KA, Kurepina NE, Lofy KH, Goldberg SV, Narita M, Nolan CM, McElroy PD, Kreiswirth BN, Cangelosi GA (2004) Expanded geographical distribution of the N family of Mycobacterium tuberculosis strains within the United States. J Clin Microbiol 42: 1064-1068. PMI: 15004054

Manca C, Reed MB, Freeman S, Mathema B, Kreiswirth B, Barry CE, 3rd, Kaplan G (2004) Differential monocyte activation underlies strain-specific Mycobacterium tuberculosis pathogenesis. Infect Immun 72: 5511-5514. PMI: 15322056

Koreen L, Ramaswamy SV, Graviss EA, Naidich S, Musser JM, Kreiswirth BN (2004) spa typing method for discriminating among Staphylococcus aureus isolates: implications for use of a single marker to detect genetic micro- and macrovariation. J Clin Microbiol 42: 792-799. PMI: 14766855

Charlebois ED, Perdreau-Remington F, Kreiswirth B, Bangsberg DR, Ciccarone D, Diep BA, Ng VL, Chansky K, Edlin BR, Chambers HF (2004) Origins of community strains of methicillin-resistant Staphylococcus aureus. Clin Infect Dis 39: 47-54. PMI: 15206052

Zhao X, Eisner W, Perl-Rosenthal N, Kreiswirth B, Drlica K (2003) Mutant prevention concentration of garenoxacin (BMS-284756) for ciprofloxacin-susceptible or -resistant Staphylococcus aureus. Antimicrob Agents Chemother 47: 1023-1027. PMI: 12604537

Wang XM, Noble L, Kreiswirth BN, Eisner W, McClements W, Jansen KU, Anderson AS (2003) Evaluation of a multilocus sequence typing system for Staphylococcus epidermidis. J Med Microbiol 52: 989-998. PMI: 14532344

Somerville GA, Said-Salim B, Wickman JM, Raffel SJ, Kreiswirth BN, Musser JM (2003) Correlation of acetate catabolism and growth yield in Staphylococcus aureus: implications for host-pathogen interactions. Infect Immun 71: 4724-4732. PMI: 12874354

Shopsin B, Mathema B, Alcabes P, Said-Salim B, Lina G, Matsuka A, Martinez J, Kreiswirth BN (2003) Prevalence of agr specificity groups among Staphylococcus aureus strains colonizing children and their guardians. J Clin Microbiol 41: 456-459. PMI: 12517893

Shopsin B, Herring S, Kreiswirth BN (2003) Hospital-acquired and community-derived: the future of MRSA? Clin Infect Dis 37: 151-152; author reply 152. PMI: 12830425

Saiman L, O'Keefe M, Graham PL, 3rd, Wu F, Said-Salim B, Kreiswirth B, LaSala A, Schlievert PM, Della-Latta P (2003) Hospital transmission of community-acquired methicillin-resistant Staphylococcus aureus among postpartum women. Clin Infect Dis 37: 1313-1319. PMI: 14583864

Saiman L, Cronquist A, Wu F, Zhou J, Rubenstein D, Eisner W, Kreiswirth BN, Della-Latta P (2003) An outbreak of methicillin-resistant Staphylococcus aureus in a neonatal intensive care unit. Infect Control Hosp Epidemiol 24: 317-321. PMI: 12785403

Said-Salim B, Mathema B, Kreiswirth BN (2003) Community-acquired methicillin-resistant Staphylococcus aureus: an emerging pathogen. Infect Control Hosp Epidemiol 24: 451-455. PMI: 12828324

Said-Salim B, Dunman PM, McAleese FM, Macapagal D, Murphy E, McNamara PJ, Arvidson S, Foster TJ, Projan SJ, Kreiswirth BN (2003) Global regulation of Staphylococcus aureus genes by Rot. J Bacteriol 185: 610-619. PMI: 12511508

Rosato AE, Kreiswirth BN, Craig WA, Eisner W, Climo MW, Archer GL (2003) mecA-blaZ corepressors in clinical Staphylococcus aureus isolates. Antimicrob Agents Chemother 47: 1460-1463. PMI: 12654694

Munsiff SS, Nivin B, Sacajiu G, Mathema B, Bifani P, Kreiswirth BN (2003) Persistence of a highly resistant strain of tuberculosis in New York City during 1990-1999. J Infect Dis 188: 356-363. PMI: 12870116

Kuo MR, Morbidoni HR, Alland D, Sneddon SF, Gourlie BB, Staveski MM, Leonard M, Gregory JS, Janjigian AD, Yee C, Musser JM, Kreiswirth B, Iwamoto H, Perozzo R, Jacobs WR, Jr., Sacchettini JC, Fidock DA (2003) Targeting tuberculosis and malaria through inhibition of Enoyl reductase: compound activity and structural data. J Biol Chem 278: 20851-20859. PMI: 12606558

Kaplan G, Post FA, Moreira AL, Wainwright H, Kreiswirth BN, Tanverdi M, Mathema B, Ramaswamy SV, Walther G, Steyn LM, Barry CE, 3rd, Bekker LG (2003) Mycobacterium tuberculosis growth at the cavity surface: a microenvironment with failed immunity. Infect Immun 71: 7099-7108. PMI: 14638800

Huard RC, Chitale S, Leung M, Lazzarini LC, Zhu H, Shashkina E, Laal S, Conde MB, Kritski AL, Belisle JT, Kreiswirth BN, Lapa e Silva JR, Ho JL (2003) The Mycobacterium tuberculosis complex-restricted gene cfp32 encodes an expressed protein that is detectable in tuberculosis patients and is positively correlated with pulmonary interleukin-10. Infect Immun 71: 6871-6883. PMI: 14638775

Filliol I, Driscoll JR, van Soolingen D, Kreiswirth BN, Kremer K, Valetudie G, Dang DA, Barlow R, Banerjee D, Bifani PJ, Brudey K, Cataldi A, Cooksey RC, Cousins DV, Dale JW, Dellagostin OA, Drobniewski F, Engelmann G, Ferdinand S, Gascoyne-Binzi D, Gordon M, Gutierrez MC, Haas WH, Heersma H, Kassa-Kelembho E, Ho ML, Makristathis A, Mammina C, Martin G, Mostrom P, Mokrousov I, Narbonne V, Narvskaya O, Nastasi A, Niobe-Eyangoh SN, Pape JW, Rasolofo-Razanamparany V, Ridell M, Rossetti ML, Stauffer F, Suffys PN, Takiff H, Texier-Maugein J, Vincent V, de Waard JH, Sola C, Rastogi N (2003) Snapshot of moving and expanding clones of Mycobacterium tuberculosis and their global distribution assessed by spoligotyping in an international study. J Clin Microbiol 41: 1963-1970. PMI: 12734235

Fey PD, Said-Salim B, Rupp ME, Hinrichs SH, Boxrud DJ, Davis CC, Kreiswirth BN, Schlievert PM (2003) Comparative molecular analysis of community- or hospital-acquired methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 47: 196-203. PMI: 12499191

Tazi L, Kreiswirth B, Carriere C, Tibayrenc M (2002) Molecular epidemiology of Mycobacterium tuberculosis and its relevance to the surveillance and control of TB: an e-debate. Infect Genet Evol 2: 153-158. PMI: 12797993

Munsiff SS, Bassoff T, Nivin B, Li J, Sharma A, Bifani P, Mathema B, Driscoll J, Kreiswirth BN (2002) Molecular epidemiology of multidrug-resistant tuberculosis, New York City, 1995-1997. Emerg Infect Dis 8: 1230-1238. PMI: 12453347

McElroy PD, Sterling TR, Driver CR, Kreiswirth B, Woodley CL, Cronin WA, Hardge DX, Shilkret KL, Ridzon R (2002) Use of DNA fingerprinting to investigate a multiyear, multistate tuberculosis outbreak. Emerg Infect Dis 8: 1252-1256. PMI: 12453351

Mathema B, Bifani PJ, Driscoll J, Steinlein L, Kurepina N, Moghazeh SL, Shashkina E, Marras SA, Campbell S, Mangura B, Shilkret K, Crawford JT, Frothingham R, Kreiswirth BN (2002) Identification and evolution of an IS6110 low-copy-number Mycobacterium tuberculosis cluster. J Infect Dis 185: 641-649. PMI: 11865421

Low DE, Muller M, Duncan CL, Willey BM, de Azavedo JC, McGeer A, Kreiswirth BN, Pong-Porter S, Bast DJ (2002) Activity of BMS-284756, a novel des-fluoro(6) quinolone, against Staphylococcus aureus, including contributions of mutations to quinolone resistance. Antimicrob Agents Chemother 46: 1119-1121. PMI: 11897603

Low DE, Kreiswirth BN, Weiss K, Willey BM (2002) Activity of GAR-936 and other antimicrobial agents against North American isolates of Staphylococcus aureus. Int J Antimicrob Agents 20: 220-222. PMI: 12385702

Gutacker MM, Smoot JC, Migliaccio CA, Ricklefs SM, Hua S, Cousins DV, Graviss EA, Shashkina E, Kreiswirth BN, Musser JM (2002) Genome-wide analysis of synonymous single nucleotide polymorphisms in Mycobacterium tuberculosis complex organisms: resolution of genetic relationships among closely related microbial strains. Genetics 162: 1533-1543. PMI: 12524330

Geng E, Kreiswirth B, Driver C, Li J, Burzynski J, DellaLatta P, LaPaz A, Schluger NW (2002) Changes in the transmission of tuberculosis in New York City from 1990 to 1999. N Engl J Med 346: 1453-1458. PMI: 12000815

Filliol I, Driscoll JR, Van Soolingen D, Kreiswirth BN, Kremer K, Valetudie G, Anh DD, Barlow R, Banerjee D, Bifani PJ, Brudey K, Cataldi A, Cooksey RC, Cousins DV, Dale JW, Dellagostin OA, Drobniewski F, Engelmann G, Ferdinand S, Gascoyne-Binzi D, Gordon M, Gutierrez MC, Haas WH, Heersma H, Kallenius G, Kassa-Kelembho E, Koivula T, Ly HM, Makristathis A, Mammina C, Martin G, Mostrom P, Mokrousov I, Narbonne V, Narvskaya O, Nastasi A, Niobe-Eyangoh SN, Pape JW, Rasolofo-Razanamparany V, Ridell M, Rossetti ML, Stauffer F, Suffys PN, Takiff H, Texier-Maugein J, Vincent V, De Waard JH, Sola C, Rastogi N (2002) Global distribution of Mycobacterium tuberculosis spoligotypes. Emerg Infect Dis 8: 1347-1349. PMI: 12453368

Dziadek J, Rajagopalan M, Parish T, Kurepina N, Greendyke R, Kreiswirth BN, Madiraju MV (2002) Mutations in the CCGTTCACA DnaA box of Mycobacterium tuberculosis oriC that abolish replication of oriC plasmids are tolerated on the chromosome. J Bacteriol 184: 3848-3855. PMI: 12081955

Driscoll JR, Bifani PJ, Mathema B, McGarry MA, Zickas GM, Kreiswirth BN, Taber HW (2002) Spoligologos: a bioinformatic approach to displaying and analyzing Mycobacterium tuberculosis data. Emerg Infect Dis 8: 1306-1309. PMI: 12453361

Bifani PJ, Mathema B, Kurepina NE, Kreiswirth BN (2002) Global dissemination of the Mycobacterium tuberculosis W-Beijing family strains. Trends Microbiol 10: 45-52. PMI: 11755085

Waddell RD, Lishimpi K, von Reyn CF, Chintu C, Baboo KS, Kreiswirth B, Talbot EA, Karagas MR (2001) Bacteremia due to Mycobacterium tuberculosis or M. bovis, Bacille Calmette-Guerin (BCG) among HIV- positive children and adults in Zambia. AIDS 15: 55-60. PMI: 11192868

Shopsin B, Kreiswirth BN (2001) Molecular epidemiology of methicillin-resistant Staphylococcus aureus. Emerg Infect Dis 7: 323-326. PMI: 11294733

Magnani J, Mathema B, Berger J, Brown S, David M, Glatt A, Pujol F, Segal-Maurer S, Riley LW, Kreiswirth BN, Sepkowitz KA (2001) Molecular epidemiology of tuberculosis among eight hospitals in New York City, 1996-1997. Int J Infect Dis 5: 126-132. PMI: 11724668

Kato-Maeda M, Bifani PJ, Kreiswirth BN, Small PM (2001) The nature and consequence of genetic variability within Mycobacterium tuberculosis. J Clin Invest 107: 533-537. PMI: 11238552

Johnson S, Brusasca P, Lyashchenko K, Spencer JS, Wiker HG, Bifani P, Shashkina E, Kreiswirth B, Harboe M, Schluger N, Gomez M, Gennaro ML (2001) Characterization of the secreted MPT53 antigen of Mycobacterium tuberculosis. Infect Immun 69: 5936-5939. PMI: 11500477

Bifani P, Mathema B, Campo M, Moghazeh S, Nivin B, Shashkina E, Driscoll J, Munsiff SS, Frothingham R, Kreiswirth BN (2001) Molecular identification of streptomycin monoresistant Mycobacterium tuberculosis related to multidrug-resistant W strain. Emerg Infect Dis 7: 842-848. PMI: 11747697

Lutfey M, Della-Latta P, Kapur V, Palumbo LA, Gurner D, Stotzky G, Brudney K, Dobkin J, Moss A, Musser JM, Kreiswirth BN (1996) Independent origin of mono-rifampin-resistant Mycobacterium tuberculosis in patients with AIDS. Am J Respir Crit Care Med 153: 837-840. PMI: 8564140

Bifani PJ, Plikaytis BB, Kapur V, Stockbauer K, Pan X, Lutfey ML, Moghazeh SL, Eisner W, Daniel TM, Kaplan MH, Crawford JT, Musser JM, Kreiswirth BN (1996) Origin and interstate spread of a New York City multidrug-resistant Mycobacterium tuberculosis clone family. JAMA 275: 452-457. PMI: 8627966

Takiff HE, Salazar L, Guerrero C, Philipp W, Huang WM, Kreiswirth B, Cole ST, Jacobs WR, Jr., Telenti A (1994) Cloning and nucleotide sequence of Mycobacterium tuberculosis gyrA and gyrB genes and detection of quinolone resistance mutations. Antimicrob Agents Chemother 38: 773-780. PMI: 8031045

Kapur V, Li LL, Iordanescu S, Hamrick MR, Wanger A, Kreiswirth BN, Musser JM (1994) Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas. J Clin Microbiol 32: 1095-1098. PMI: 8027320

Kreiswirth B, Kornblum J, Arbeit RD, Eisner W, Maslow JN, McGeer A, Low DE, Novick RP (1993) Evidence for a clonal origin of methicillin resistance in Staphylococcus aureus. Science 259: 227-230. PMI: 8093647

Frieden TR, Munsiff SS, Low DE, Willey BM, Williams G, Faur Y, Eisner W, Warren S, Kreiswirth B (1993) Emergence of vancomycin-resistant enterococci in New York City. Lancet 342: 76-79. PMI: 8100912






C.V.

Ph.D. Hamilton College, B.A., 1976; University of Western Ontario, Research Fellow, 1977-1979; New York University, Ph.D., 1986; PHRI, Post Doc., 1986-1988; PHRI, Research Scientist, 1988-1990; New York City Department of Health Phage and Typing and Antibiotic Susceptibility Testing Laboratory, Chief, 1990-1992; PHRI TB Center, Director, 1992-present; NYU School of Medicine, Adjunct Assistant Professor of Medicine, 1994-present.


 
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