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Scientific Overview Research Interest Summary Principal Investigators    Yuri Bushkin, Ph.D.
   Theresa Chang, Ph.D.
   Neeraj Chauhan, Ph.D.
   Véronique Dartois, Ph.D.
   Karl Drlica, Ph.D.
   David Dubnau, Ph.D.
   Eliseo A. Eugenin, Ph.D.
   Marila Gennaro, M.D.
   Gilla Kaplan, Ph.D.
   Fred Kramer, Ph.D.
   Barry Kreiswirth, Ph.D.
   Min Lu, Ph.D.
   Leonard Mindich, Ph.D.
   Arkady Mustaev, Ph.D.
   Jyothi Nagajyothi, Ph.D.
   David Perlin, Ph.D.
   Richard Pine, Ph.D.
   Abraham Pinter, Ph.D.
   Marcela Rodriguez, Ph.D.
   Selvakumar Subbian, Ph.D.
   Sanjay Tyagi, Ph.D.
   Christopher Vinnard, M.D.
   Chaoyang Xue, Ph.D.
   Xilin Zhao, Ph.D.

   Research Faculty
   Liang Chen, Ph.D.
   Eugenie Dubnau, Ph.D.
   Jeanette Hahn, Ph.D.
   Salvatore Marras, Ph.D.
   Lanbo Shi, Ph.D.
   Yanan Zhao, Ph.D.

Emeritus Faculty Recent Publications
 
Liang Chen, Ph.D.

Research Summary  |  Selected Publications  |  C.V.
 

Public Health Research Institute Center and Department of Medicine
New Jersey Medical School - Rutgers, The State University of New Jersey
225 Warren Street
Newark, New Jersey 07103

Phone: (973) 854-3242
e-mail: chen11@njms.rutgers.edu



Research Summary

Dr. Chen studies the molecular epidemiology of antimicrobial resistance and infectious disease, with particular focus on carbapenem-resistant Enterobacteriaceae (CRE) and Methicillin-resistant Staphylococcus aureus (MRSA).

Currently, Dr. Chen is seeking to decipher the molecular evolution of the emergence of CRE. CRE have been described as “nightmare bacteria”, and been classified as an “urgent threat” by CDC. CRE have increased over the past decade and grown resistance to even the most powerful antibiotics. The worldwide dissemination of CRE is predominately associated with a single K. pneumoniae clone, ST258. Using the whole genomic analysis, Dr. Chen and his colleagues have revealed that the ST258 is a hybrid strain that originated as a large chromosomal recombination. Meanwhile, this superclone is under significantly diversification, primarily due to the recombination of antigen encoded capsular polysaccharide synthesis (cps) region and plasmid acquisition. Thus far, they have sequenced over 300 CRE isolates, including the first two completely closed ST258 genomes; and have completely characterized over 50 carbapenem-resistant plasmids.

A major concern about CRE is that resistance targets are primarily carried on plasmids that can easily transfer the resistance to other bacteria. Another aspect of Dr. Chen’s work is to develop robust molecular detection platforms to track the dissemination of the life threatening bacteria, and more importantly, to explore CRE transmission from the traditional “strain epidemiology” to “plasmid epidemiology”. Using WGS technique and comparative genomic analysis, Dr. Chen has identified and characterized the epidemic drug-resistant plasmids prevailing in the epicenter of this epidemic (NY/NJ) and developed robust molecular tools to rapidly track the spread of these resistance plasmids.






Selected Publications


Yu H, Qu F, Shan B, Huang B, Jia W, Chen C, Li A, Miao M, Zhang X, Bao C, Xu Y, Chavda KD, Tang YW, Kreiswirth BN, Du H, Chen L (2016) Detection of mcr-1 colistin resistance gene in carbapenem-resistant Enterobacteriaceae (CRE) from different hospitals in China. Antimicrob Agents Chemother. PMI: 27216058

Xie X, Zhang H, Zheng Y, Li A, Wang M, Zhou H, Zhu X, Schneider Z, Chen L, Kreiswirth BN, Du H (2016) RpoE is a Putative Antibiotic Resistance Regulator of Salmonella enteric Serovar Typhi. Curr Microbiol 72: 457-464. PMI: 26742769

Prasad N, Labaze G, Kopacz J, Chwa S, Platis D, Pan CX, Russo D, LaBombardi VJ, Osorio G, Pollack S, Kreiswirth BN, Chen L, Urban C, Segal-Maurer S (2016) Asymptomatic rectal colonization with carbapenem-resistant Enterobacteriaceae and Clostridium difficile among residents of a long-term care facility in New York City. Am J Infect Control 44: 525-532. PMI: 26796684

Ocampo AM, Chen L, Cienfuegos AV, Roncancio G, Chavda KD, Kreiswirth BN, Jimenez JN (2016) A Two-Year Surveillance in Five Colombian Tertiary Care Hospitals Reveals High Frequency of Non-CG258 Clones of Carbapenem-Resistant Klebsiella pneumoniae with Distinct Clinical Characteristics. Antimicrob Agents Chemother 60: 332-342. PMI: 26503660

Lynch T, Chen L, Peirano G, Gregson DB, Church DL, Conly J, Kreiswirth BN, Pitout J (2016) Molecular evolution of Klebsiella pneumoniae ST278 harboring blaNDM-7 involved in nosocomial transmission. J Infect Dis. PMI: 27284091

Li A, Yang Y, Miao M, Chavda KD, Mediavilla JR, Xie X, Feng P, Tang YW, Kreiswirth BN, Chen L, Du H (2016) Complete Sequences of mcr-1-Harboring Plasmids from Extended-Spectrum-beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae. Antimicrob Agents Chemother 60: 4351-4354. PMI: 27090180

Kobayashi SD, Porter AR, Dorward DW, Brinkworth AJ, Chen L, Kreiswirth BN, DeLeo FR (2016) Phagocytosis and Killing of Carbapenem-Resistant ST258 Klebsiella pneumoniae by Human Neutrophils. J Infect Dis 213: 1615-1622. PMI: 26768252

Evans SR, Pennello G, Pantoja-Galicia N, Jiang H, Hujer AM, Hujer KM, Manca C, Hill C, Jacobs MR, Chen L, Patel R, Kreiswirth BN, Bonomo RA (2016) Benefit-risk Evaluation for Diagnostics: A Framework (BED-FRAME). Clin Infect Dis. PMI: 27193750

Evans SR, Hujer AM, Jiang H, Hujer KM, Hall T, Marzan C, Jacobs MR, Sampath R, Ecker DJ, Manca C, Chavda K, Zhang P, Fernandez H, Chen L, Mediavilla JR, Hill CB, Perez F, Caliendo AM, Fowler VG, Jr., Chambers HF, Kreiswirth BN, Bonomo RA (2016) Rapid Molecular Diagnostics, Antibiotic Treatment Decisions, and Developing Approaches to Inform Empiric Therapy: PRIMERS I and II. Clin Infect Dis 62: 181-189. PMI: 26409063

Du H, Chen L, Tang YW, Kreiswirth BN (2016) Emergence of the mcr-1 colistin resistance gene in carbapenem-resistant Enterobacteriaceae. Lancet Infect Dis 16: 287-288. PMI: 26842776

Du H, Chen L, Chavda KD, Pandey R, Zhang H, Xie X, Tang YW, Kreiswirth BN (2016) Genomic Characterization of Enterobacter cloacae Isolates from China That Coproduce KPC-3 and NDM-1 Carbapenemases. Antimicrob Agents Chemother 60: 2519-2523. PMI: 26787700

Chen L, Peirano G, Lynch T, Chavda KD, Gregson DB, Church DL, Conly J, Kreiswirth BN, Pitout JD (2016) Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing blaNDM-7 in Enterobacteriaceae from Calgary, Canada. Antimicrob Agents Chemother 60: 1258-1263. PMI: 26643346

Chavda KD, Chen L, Jacobs MR, Bonomo RA, Kreiswirth BN (2016) Molecular Diversity and Plasmid Analysis of KPC-Producing Escherichia coli. Antimicrob Agents Chemother 60: 4073-4081. PMI: 27114279

Buehrle DJ, Shields RK, Chen L, Hao B, Press EG, Alkrouk A, Potoski BA, Kreiswirth BN, Clancy CJ, Nguyen MH (2016) Evaluation of the In Vitro Activity of Ceftazidime-Avibactam and Ceftolozane-Tazobactam against Meropenem-Resistant Pseudomonas aeruginosa Isolates. Antimicrob Agents Chemother 60: 3227-3231. PMI: 26976862

Xiong H, Carter RA, Leiner IM, Tang YW, Chen L, Kreiswirth BN, Pamer EG (2015) Distinct Contributions of Neutrophils and CCR2+ Monocytes to Pulmonary Clearance of Different Klebsiella pneumoniae Strains. Infect Immun 83: 3418-3427. PMI: 26056382

Shields RK, Nguyen MH, Potoski BA, Press EG, Chen L, Kreiswirth BN, Clarke LG, Eschenauer GA, Clancy CJ (2015) Doripenem MICs and ompK36 porin genotypes of sequence type 258, KPC-producing Klebsiella pneumoniae may predict responses to carbapenem-colistin combination therapy among patients with bacteremia. Antimicrob Agents Chemother 59: 1797-1801. PMI: 25534733

Shields RK, Clancy CJ, Hao B, Chen L, Press EG, Iovine NM, Kreiswirth BN, Nguyen MH (2015) Effects of Klebsiella pneumoniae carbapenemase subtypes, extended-spectrum beta-lactamases, and porin mutations on the in vitro activity of ceftazidime-avibactam against carbapenem-resistant K. pneumoniae. Antimicrob Agents Chemother 59: 5793-5797. PMI: 26169413

LaBombardi VJ, Urban CM, Kreiswirth BN, Chen L, Osorio G, Kopacz J, Labaze G, Segal-Maurer S (2015) Evaluation of Remel Spectra CRE Agar for Detection of Carbapenem-Resistant Bacteria from Rectal Swabs Obtained from Residents of a Long-Term-Care Facility. J Clin Microbiol 53: 2823-2826. PMI: 26085613

Hu Y, Mathema B, Zhao Q, Chen L, Lu W, Wang W, Kreiswirth B, Xu B (2015) Acquisition of second-line drug resistance and extensive drug resistance during recent transmission of Mycobacterium tuberculosis in rural China. Clin Microbiol Infect 21: 1093.e1099-1093.e1018. PMI: 26348262

Chen L, Chavda KD, DeLeo FR, Bryant KA, Jacobs MR, Bonomo RA, Kreiswirth BN (2015) Genome Sequence of a Klebsiella pneumoniae Sequence Type 258 Isolate with Prophage-Encoded K. pneumoniae Carbapenemase. Genome Announc 3. PMI: 26089425

Chen L, Al Laham N, Chavda KD, Mediavilla JR, Jacobs MR, Bonomo RA, Kreiswirth BN (2015) First report of an OXA-48-producing multidrug-resistant Proteus mirabilis strain from Gaza, Palestine. Antimicrob Agents Chemother 59: 4305-4307. PMI: 25896692

Chavda KD, Chen L, Jacobs MR, Rojtman AD, Bonomo RA, Kreiswirth BN (2015) Complete sequence of a bla(KPC)-harboring cointegrate plasmid isolated from Escherichia coli. Antimicrob Agents Chemother 59: 2956-2959. PMI: 25753632

Brinkworth AJ, Hammer CH, Olano LR, Kobayashi SD, Chen L, Kreiswirth BN, DeLeo FR (2015) Identification of Outer Membrane and Exoproteins of Carbapenem-Resistant Multilocus Sequence Type 258 Klebsiella pneumoniae. PLoS One 10: e0123219. PMI: 25893665

Bayer AS, Mishra NN, Chen L, Kreiswirth BN, Rubio A, Yang SJ (2015) Frequency and Distribution of Single-Nucleotide Polymorphisms within mprF in Methicillin-Resistant Staphylococcus aureus Clinical Isolates and Their Role in Cross-Resistance to Daptomycin and Host Defense Antimicrobial Peptides. Antimicrob Agents Chemother 59: 4930-4937. PMI: 26055370

Al Laham N, Mediavilla JR, Chen L, Abdelateef N, Elamreen FA, Ginocchio CC, Pierard D, Becker K, Kreiswirth BN (2015) MRSA clonal complex 22 strains harboring toxic shock syndrome toxin (TSST-1) are endemic in the primary hospital in Gaza, Palestine. PLoS One 10: e0120008. PMI: 25781188

Abdelhady W, Chen L, Bayer AS, Seidl K, Yeaman MR, Kreiswirth BN, Xiong YQ (2015) Early agr activation correlates with vancomycin treatment failure in multi-clonotype MRSA endovascular infections. J Antimicrob Chemother 70: 1443-1452. PMI: 25564565

Rosenthal ME, Mediavilla J, Chen L, Sonnenfeld J, Pierce L, Shannon A, Boucher H, Pearlmutter M, Kreiswirth B, Kuo YH, Previl H, Rojtman A (2014) Molecular epidemiology of Staphylococcus aureus in post-earthquake northern Haiti. Int J Infect Dis 29: 146-151. PMI: 25449249

Perez F, Hujer AM, Marshall SH, Ray AJ, Rather PN, Suwantarat N, Dumford D, 3rd, O'Shea P, Domitrovic TN, Salata RA, Chavda KD, Chen L, Kreiswirth BN, Vila AJ, Haussler S, Jacobs MR, Bonomo RA (2014) Extensively drug-resistant pseudomonas aeruginosa isolates containing blaVIM-2 and elements of Salmonella genomic island 2: a new genetic resistance determinant in Northeast Ohio. Antimicrob Agents Chemother 58: 5929-5935. PMI: 25070102

Diago-Navarro E, Chen L, Passet V, Burack S, Ulacia-Hernando A, Kodiyanplakkal RP, Levi MH, Brisse S, Kreiswirth BN, Fries BC (2014) Carbapenem-resistant Klebsiella pneumoniae exhibit variability in capsular polysaccharide and capsule associated virulence traits. J Infect Dis 210: 803-813. PMI: 24634498

Deleo FR, Chen L, Porcella SF, Martens CA, Kobayashi SD, Porter AR, Chavda KD, Jacobs MR, Mathema B, Olsen RJ, Bonomo RA, Musser JM, Kreiswirth BN (2014) Molecular dissection of the evolution of carbapenem-resistant multilocus sequence type 258 Klebsiella pneumoniae. Proc Natl Acad Sci U S A 111: 4988-4993. PMI: 24639510

Dara JS, Chen L, Levi MH, Kreiswirth BN, Pellett Madan R (2014) Microbiological and Genetic Characterization of Carbapenem-Resistant Klebsiella pneumoniae Isolated From Pediatric Patients. J Pediatric Infect Dis Soc 3: e10-14. PMI: 24567846

Clancy CJ, Hao B, Shields RK, Chen L, Perlin DS, Kreiswirth BN, Nguyen MH (2014) Doripenem, gentamicin, and colistin, alone and in combinations, against gentamicin-susceptible, KPC-producing Klebsiella pneumoniae strains with various ompK36 genotypes. Antimicrob Agents Chemother 58: 3521-3525. PMI: 24566172

Chen L, Mathema B, Pitout JD, DeLeo FR, Kreiswirth BN (2014) Epidemic Klebsiella pneumoniae ST258 is a hybrid strain. MBio 5: e01355-01314. PMI: 24961694

Chen L, Mathema B, Chavda KD, DeLeo FR, Bonomo RA, Kreiswirth BN (2014) Carbapenemase-producing Klebsiella pneumoniae: molecular and genetic decoding. Trends Microbiol 22: 686-696. PMI: 25304194

Chen L, Hu H, Chavda KD, Zhao S, Liu R, Liang H, Zhang W, Wang X, Jacobs MR, Bonomo RA, Kreiswirth BN (2014) Complete sequence of a KPC-producing IncN multidrug-resistant plasmid from an epidemic Escherichia coli sequence type 131 strain in China. Antimicrob Agents Chemother 58: 2422-2425. PMI: 24395232

Chen L, Chavda KD, Melano RG, Jacobs MR, Koll B, Hong T, Rojtman AD, Levi MH, Bonomo RA, Kreiswirth BN (2014) Comparative genomic analysis of KPC-encoding pKpQIL-like plasmids and their distribution in New Jersey and New York Hospitals. Antimicrob Agents Chemother 58: 2871-2877. PMI: 24614371

Chen L, Chavda KD, Melano RG, Hong T, Rojtman AD, Jacobs MR, Bonomo RA, Kreiswirth BN (2014) Molecular survey of the dissemination of two blaKPC-harboring IncFIA plasmids in New Jersey and New York hospitals. Antimicrob Agents Chemother 58: 2289-2294. PMI: 24492370

Chen L, Chavda KD, Findlay J, Peirano G, Hopkins K, Pitout JD, Bonomo RA, Woodford N, DeLeo FR, Kreiswirth BN (2014) Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains. Antimicrob Agents Chemother 58: 4196-4199. PMI: 24733470

Bremmer DN, Clancy CJ, Press EG, Almaghrabi R, Chen L, Doi Y, Nguyen MH, Shields RK (2014) KPC-producing Klebsiella pneumoniae strains that harbor AAC(6')-Ib exhibit intermediate resistance to amikacin. Antimicrob Agents Chemother 58: 7597-7600. PMI: 25288089

Almaghrabi R, Clancy CJ, Doi Y, Hao B, Chen L, Shields RK, Press EG, Iovine NM, Townsend BM, Wagener MM, Kreiswirth B, Nguyen MH (2014) Carbapenem-resistant Klebsiella pneumoniae strains exhibit diversity in aminoglycoside-modifying enzymes, which exert differing effects on plazomicin and other agents. Antimicrob Agents Chemother 58: 4443-4451. PMI: 24867988

Satlin MJ, Jenkins SG, Chen L, Helfgott D, Feldman EJ, Kreiswirth BN, Schuetz AN (2013) Septic shock caused by Klebsiella pneumoniae carbapenemase-producing Enterobacter gergoviae in a neutropenic patient with leukemia. J Clin Microbiol 51: 2794-2796. PMI: 23761145

Satlin MJ, Calfee DP, Chen L, Fauntleroy KA, Wilson SJ, Jenkins SG, Feldman EJ, Roboz GJ, Shore TB, Helfgott DC, Soave R, Kreiswirth BN, Walsh TJ (2013) Emergence of carbapenem-resistant Enterobacteriaceae as causes of bloodstream infections in patients with hematologic malignancies. Leuk Lymphoma 54: 799-806. PMI: 22916826

Perez F, Rudin SD, Marshall SH, Coakley P, Chen L, Kreiswirth BN, Rather PN, Hujer AM, Toltzis P, van Duin D, Paterson DL, Bonomo RA (2013) OqxAB, a quinolone and olaquindox efflux pump, is widely distributed among multidrug-resistant Klebsiella pneumoniae isolates of human origin. Antimicrob Agents Chemother 57: 4602-4603. PMI: 23817374

Mishra NN, Yang SJ, Chen L, Muller C, Saleh-Mghir A, Kuhn S, Peschel A, Yeaman MR, Nast CC, Kreiswirth BN, Cremieux AC, Bayer AS (2013) Emergence of daptomycin resistance in daptomycin-naive rabbits with methicillin-resistant Staphylococcus aureus prosthetic joint infection is associated with resistance to host defense cationic peptides and mprF polymorphisms. PLoS One 8: e71151. PMI: 23990934

Jimenez JN, Ocampo AM, Vanegas JM, Rodriguez EA, Mediavilla JR, Chen L, Muskus CE, Velez LA, Rojas C, Restrepo AV, Garces C, Kreiswirth BN, Correa MM (2013) A comparison of methicillin-resistant and methicillin-susceptible Staphylococcus aureus reveals no clinical and epidemiological but molecular differences. Int J Med Microbiol 303: 76-83. PMI: 23369303

Hong JH, Clancy CJ, Cheng S, Shields RK, Chen L, Doi Y, Zhao Y, Perlin DS, Kreiswirth BN, Nguyen MH (2013) Characterization of porin expression in Klebsiella pneumoniae Carbapenemase (KPC)-producing K. pneumoniae identifies isolates most susceptible to the combination of colistin and carbapenems. Antimicrob Agents Chemother 57: 2147-2153. PMI: 23459476

Clancy CJ, Chen L, Shields RK, Zhao Y, Cheng S, Chavda KD, Hao B, Hong JH, Doi Y, Kwak EJ, Silveira FP, Abdel-Massih R, Bogdanovich T, Humar A, Perlin DS, Kreiswirth BN, Hong Nguyen M (2013) Epidemiology and molecular characterization of bacteremia due to carbapenem-resistant Klebsiella pneumoniae in transplant recipients. Am J Transplant 13: 2619-2633. PMI: 24011185

Clancy CJ, Chen L, Hong JH, Cheng S, Hao B, Shields RK, Farrell AN, Doi Y, Zhao Y, Perlin DS, Kreiswirth BN, Nguyen MH (2013) Mutations of the ompK36 porin gene and promoter impact responses of sequence type 258, KPC-2-producing Klebsiella pneumoniae strains to doripenem and doripenem-colistin. Antimicrob Agents Chemother 57: 5258-5265. PMI: 23939888

Chen L, Chavda KD, Solanki M, Mediavilla JR, Mathema B, Schlievert PM, Kreiswirth BN (2013) Genetic variation among Panton-Valentine leukocidin-encoding bacteriophages in Staphylococcus aureus clonal complex 30 strains. J Clin Microbiol 51: 914-919. PMI: 23284024

Chen L, Chavda KD, Melano RG, Jacobs MR, Levi MH, Bonomo RA, Kreiswirth BN (2013) Complete sequence of a bla(KPC-2)-harboring IncFII(K1) plasmid from a Klebsiella pneumoniae sequence type 258 strain. Antimicrob Agents Chemother 57: 1542-1545. PMI: 23295924

Chen L, Chavda KD, Fraimow HS, Mediavilla JR, Melano RG, Jacobs MR, Bonomo RA, Kreiswirth BN (2013) Complete nucleotide sequences of blaKPC-4- and blaKPC-5-harboring IncN and IncX plasmids from Klebsiella pneumoniae strains isolated in New Jersey. Antimicrob Agents Chemother 57: 269-276. PMI: 23114770

Chen L, Chavda KD, Al Laham N, Melano RG, Jacobs MR, Bonomo RA, Kreiswirth BN (2013) Complete nucleotide sequence of a blaKPC-harboring IncI2 plasmid and its dissemination in New Jersey and New York hospitals. Antimicrob Agents Chemother 57: 5019-5025. PMI: 23896467

Xiong YQ, Hady WA, Bayer AS, Chen L, Kreiswirth BN, Yang SJ (2012) Telavancin in therapy of experimental aortic valve endocarditis in rabbits due to daptomycin-nonsusceptible methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 56: 5528-5533. PMI: 22890759

Weisenberg SA, Mediavilla JR, Chen L, Alexander EL, Rhee KY, Kreiswirth BN, Jenkins SG (2012) Extended spectrum beta-lactamase-producing Enterobacteriaceae in international travelers and non-travelers in New York City. PLoS One 7: e45141. PMI: 23028808

Wang SH, Khan Y, Hines L, Mediavilla JR, Zhang L, Chen L, Hoet A, Bannerman T, Pancholi P, Robinson DA, Kreiswirth BN, Stevenson KB (2012) Methicillin-resistant Staphylococcus aureus sequence type 239-III, Ohio, USA, 2007-2009. Emerg Infect Dis 18: 1557-1565. PMI: 23018025

Smyth DS, Kafer JM, Wasserman GA, Velickovic L, Mathema B, Holzman RS, Knipe TA, Becker K, von Eiff C, Peters G, Chen L, Kreiswirth BN, Novick RP, Shopsin B (2012) Nasal carriage as a source of agr-defective Staphylococcus aureus bacteremia. J Infect Dis 206: 1168-1177. PMI: 22859823

Mediavilla JR, Chen L, Uhlemann AC, Hanson BM, Rosenthal M, Stanak K, Koll B, Fries BC, Armellino D, Schilling ME, Weiss D, Smith TC, Lowy FD, Kreiswirth BN (2012) Methicillin-susceptible Staphylococcus aureus ST398, New York and New Jersey, USA. Emerg Infect Dis 18: 700-702. PMI: 22469250

Mediavilla JR, Chen L, Mathema B, Kreiswirth BN (2012) Global epidemiology of community-associated methicillin resistant Staphylococcus aureus (CA-MRSA). Curr Opin Microbiol 15: 588-595. PMI: 23044073

Jimenez JN, Ocampo AM, Vanegas JM, Rodriguez EA, Mediavilla JR, Chen L, Muskus CE, Velez LA, Rojas C, Restrepo AV, Ospina S, Garces C, Franco L, Bifani P, Kreiswirth BN, Correa MM (2012) CC8 MRSA strains harboring SCCmec type IVc are predominant in Colombian hospitals. PLoS One 7: e38576. PMI: 22745670

Chen L, Shopsin B, Zhao Y, Smyth D, Wasserman GA, Fang C, Liu L, Kreiswirth BN (2012) Real-time nucleic acid sequence-based amplification assay for rapid detection and quantification of agr functionality in clinical Staphylococcus aureus isolates. J Clin Microbiol 50: 657-661. PMI: 22219302

Chen L, Chavda KD, Mediavilla JR, Zhao Y, Fraimow HS, Jenkins SG, Levi MH, Hong T, Rojtman AD, Ginocchio CC, Bonomo RA, Kreiswirth BN (2012) Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone. Antimicrob Agents Chemother 56: 3444-3447. PMI: 22450983

Chen L, Chavda KD, Mediavilla JR, Jacobs MR, Levi MH, Bonomo RA, Kreiswirth BN (2012) Partial excision of blaKPC from Tn4401 in carbapenem-resistant Klebsiella pneumoniae. Antimicrob Agents Chemother 56: 1635-1638. PMI: 22203593

Seidl K, Chen L, Bayer AS, Hady WA, Kreiswirth BN, Xiong YQ (2011) Relationship of agr expression and function with virulence and vancomycin treatment outcomes in experimental endocarditis due to methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 55: 5631-5639. PMI: 21968365

Mishra NN, McKinnell J, Yeaman MR, Rubio A, Nast CC, Chen L, Kreiswirth BN, Bayer AS (2011) In vitro cross-resistance to daptomycin and host defense cationic antimicrobial peptides in clinical methicillin-resistant Staphylococcus aureus isolates. Antimicrob Agents Chemother 55: 4012-4018. PMI: 21709105

DeLeo FR, Kennedy AD, Chen L, Bubeck Wardenburg J, Kobayashi SD, Mathema B, Braughton KR, Whitney AR, Villaruz AE, Martens CA, Porcella SF, McGavin MJ, Otto M, Musser JM, Kreiswirth BN (2011) Molecular differentiation of historic phage-type 80/81 and contemporary epidemic Staphylococcus aureus. Proc Natl Acad Sci U S A 108: 18091-18096. PMI: 22025717

Chen L, Mediavilla JR, Endimiani A, Rosenthal ME, Zhao Y, Bonomo RA, Kreiswirth BN (2011) Multiplex real-time PCR assay for detection and classification of Klebsiella pneumoniae carbapenemase gene (bla KPC) variants. J Clin Microbiol 49: 579-585. PMI: 21123529

Chatterjee SS, Chen L, Joo HS, Cheung GY, Kreiswirth BN, Otto M (2011) Distribution and regulation of the mobile genetic element-encoded phenol-soluble modulin PSM-mec in methicillin-resistant Staphylococcus aureus. PLoS One 6: e28781. PMI: 22174895

Kennedy AD, Porcella SF, Martens C, Whitney AR, Braughton KR, Chen L, Craig CT, Tenover FC, Kreiswirth BN, Musser JM, DeLeo FR (2010) Complete nucleotide sequence analysis of plasmids in strains of Staphylococcus aureus clone USA300 reveals a high level of identity among isolates with closely related core genome sequences. J Clin Microbiol 48: 4504-4511. PMI: 20943864

Ionescu R, Mediavilla JR, Chen L, Grigorescu DO, Idomir M, Kreiswirth BN, Roberts RB (2010) Molecular characterization and antibiotic susceptibility of Staphylococcus aureus from a multidisciplinary hospital in Romania. Microb Drug Resist 16: 263-272. PMI: 21034225

Chen L, Mediavilla JR, Smyth DS, Chavda KD, Ionescu R, Roberts RB, Robinson DA, Kreiswirth BN (2010) Identification of a novel transposon (Tn6072) and a truncated staphylococcal cassette chromosome mec element in methicillin-resistant Staphylococcus aureus ST239. Antimicrob Agents Chemother 54: 3347-3354. PMI: 20479198

Yoshizumi S, Zhang Y, Yamaguchi Y, Chen L, Kreiswirth BN, Inouye M (2009) Staphylococcus aureus YoeB homologues inhibit translation initiation. J Bacteriol 191: 5868-5872. PMI: 19581360

Queck SY, Khan BA, Wang R, Bach TH, Kretschmer D, Chen L, Kreiswirth BN, Peschel A, Deleo FR, Otto M (2009) Mobile genetic element-encoded cytolysin connects virulence to methicillin resistance in MRSA. PLoS Pathog 5: e1000533. PMI: 19649313

Chen L, Mediavilla JR, Oliveira DC, Willey BM, de Lencastre H, Kreiswirth BN (2009) Multiplex real-time PCR for rapid Staphylococcal cassette chromosome mec typing. J Clin Microbiol 47: 3692-3706. PMI: 19726600

Olsen RJ, Burns KM, Chen L, Kreiswirth BN, Musser JM (2008) Severe necrotizing fasciitis in a human immunodeficiency virus-positive patient caused by methicillin-resistant Staphylococcus aureus. J Clin Microbiol 46: 1144-1147. PMI: 18199782

Kennedy AD, Otto M, Braughton KR, Whitney AR, Chen L, Mathema B, Mediavilla JR, Byrne KA, Parkins LD, Tenover FC, Kreiswirth BN, Musser JM, DeLeo FR (2008) Epidemic community-associated methicillin-resistant Staphylococcus aureus: recent clonal expansion and diversification. Proc Natl Acad Sci U S A 105: 1327-1332. PMI: 18216255

Diep BA, Palazzolo-Ballance AM, Tattevin P, Basuino L, Braughton KR, Whitney AR, Chen L, Kreiswirth BN, Otto M, DeLeo FR, Chambers HF (2008) Contribution of Panton-Valentine leukocidin in community-associated methicillin-resistant Staphylococcus aureus pathogenesis. PLoS One 3: e3198. PMI: 18787708

Zhao YN, Perlin DS, Park S, Jiang RJ, Chen L, Chen Y, Gardiner R, Jiang QW (2006) FDJS03 isolates causing an outbreak of aseptic meningitis in China that evolved from a distinct Echovirus 30 lineage imported from countries of the Commonwealth of Independent States. J Clin Microbiol 44: 4142-4148. PMI: 16957029

Zhao YN, Jiang QW, Jiang RJ, Chen L, Perlin DS (2005) Echovirus 30, Jiangsu Province, China. Emerg Infect Dis 11: 562-567. PMI: 15829194






C.V.


Education

Fudan University, Shanghai, China, Ph.D. 2006.


Professional experience

2007 - 2013   Research Associate, PHRI, New Jersey Medical School, UMDNJ, Newark, NJ, USA.
2013 - 2015   Research Associate, PHRI, New Jersey Medical School, Rutgers University, Newark, NJ, USA.
Present          Assistant Professor, PHRI, New Jersey Medical School, Rutgers University, Newark, NJ, USA.


 
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